Identification Of Unknown Bacteria Lab Quizlet



Essay Unknown Microbiology Lab Report. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. Answer the following questions in the spaces provided. for experimental purposes), we have several ways of making the uptake of DNA by E. Record the microscopic appearance of cells from each culture. Case Study #1 from Lab 14: Unknown #1. Axial filament contraction occurs in spirochetes. This shockingly minimal percentage lends credence to the idea that increasing humankind's knowledge of unknown bacteria could have great future benefits. Microbiology. It is important to understand why a person would want to identify between different bacteria. The following tests were performed on the gram-­‐positive bacteria: 1. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. You will receive an unknown bacterial culture (a gram positive, and a gram negative species, mixed) in March that you must identify, for 60 points. Each clue will offer possibility to solve the puzzle. Many bacteria can be identified in the environment by inspection or using genetic techniques, but attempts to isolate and grow them in artificial culture has been unsuccessful. Incubate for at least 48 hrs. To obtain well-isolated colonies, it is essential to disperse the inoculum (sample) on the surface of an enriched agar plate so that individual bacteria are well separated from each other. Identifying The Unknown Bacterium 12 Biology Essay. In Session 2, the team will record the results and establish a key to help identify unknown bacteria in future lab exercises. MICROBIOLOGY UNKNOWN LAB REPORT. This project was created with Explain Everything ™ Interactive Whiteboard for iPad. Identifying bacteria can lead to many new discoveries such as new species or the evolution of a known species. The Microbiology Laboratory Guidebook (MLG) contains test methods used by the FSIS Field Service Laboratories to support FSIS regulatory activities. Most bacteria collected in your environment will not be harmful. Lab Report 2. The goal of this review is to describe not only the mechanism and limits of bacterial 16S rRNA gene sequence analysis but also the impact and potential contribution that the 16S rRNA gene sequence analysis can make to the understanding of clinical microbiology and infectious diseases. Extract of sample Isolation and identification of unknown bacteria. UNKNOWN LAB REPORT. For accuate identification of Enterobacteriaceae members, you may use advanced identification from this page. He is currently working to establish a similar biomedical research group focusing on drug development at SINTEF. By scooping the guts out of bacteria and refilling them with an expansive fluid. Use standard laboratory procedures, like cell staining, culturing and DNA sequencing to further narrow down your identification. Medical Laboratory Medical Science Medical School Bacillus Subtilis Ap Biology Molecular Biology Infection Control Phlebotomy Gram Negative Bacteria. We are starting our "unknown" lab this week and we each will get 2 types of bacteria that we need to identify on our own. Joan McKearnan. Bacteria are microorganisms that grow everywhere. Sayantan Mondal [pgt] Dept. Identification of Unknown Solutions INTRODUCTION In this experiment, the student will determine if a chemical reaction has taken place when two solutions are combined, describe the chemical reaction, and use this information and logic to determine the identity of ten unknown solutions. Determining the nutritional and metabolic capabilities of a bacterial isolate is the most common approach used for deter- mining the genus and species of an organism. on StudyBlue. Sequencing/Genotyping Primer Design. Microbiology Lab, flow chart for gram-staining and cocci species. In fact, viral specificity is just as specific as an enzyme-substrate reaction or an antigen-antibody reaction. In our lab, identification of unknown bacteria is divided into 3 projects; EU, OU and PCR-ID projects. Unknown tube number 114 was obtained. The purpose of the lab is to familiarize you with the science and techniques used to identify different types of bacteria based on their DNA sequence. The compound was identified to be N-Phenysuccinimide, because it had a melting range of 154. Unknown Number 115. When favored nutrients are exhausted, some bacteria may become motile to seek out nutrients, or they may produce enzymes to exploit alternative resources. Remove the cap of the nutrient agar slant. coli strains Characteristics of E. This is due to the fact that there are only a few basic shapes and physical features commonly seen in the prokaryotic world. Acid-fast Stain A. UNKNOWN LAB REPORT (Bacillus subtilis)Josie Behr. This medium contains a small amount of KNO3 (potassium nitrate). Acid Fast Stain You will then prepare a smear for an Acid-fast stain. Microbiology Unknown Project - 2014. cerebrospinal fluid (used for the culture of mycobacteria, fungi, bacteria, or bacterial antigen testing) Submit a separate sterile screw-capped tube containing at least 0. This activity consists of providing students with microbial cultures and running biochemical assays to identify the organisms. Other than that, being able to isolating and identify bacteria also is the basic knowledge and requirement for lab study, which requires high accuracy. Look that number up in the Codebook and identify your unknown. When we are transforming DNA in the laboratory (i. The smear will include your unknown and both a (+) and (-) control. Ever wonder what that contaminant in your culture is? Need to accurately identify an environmental isolate? Overview. This virtual lab will familiarize you with the science and techniques used to identify different types of bacteria based on their DNA sequences. Iwen, PhD, Associate Director, NPHL For more than 100 years, Robert Koch’s postulate that required in part the cultivation of a pathogen to show a disease/pathogen relationship, was seldom questioned and was considered the basic standard used in clinical diagnostics. The goal of this lab report is 1) to demonstrate comprehension of the methods and lab techniques learned during the semester 2) to explain the tests performed on each isolated unknown that led to the identification of each unknown 3) and to give a background on the characteristics, pathogenicity and some uses of one of the identified unknowns. In our lab, identification of unknown bacteria is divided into 3 projects; EU, OU and PCR-ID projects. If you have an unknown bacteria and you want to identify it, you'll typically perform a gram stain and then observe the colony appearance and the individual features. Observations: The unknown was yellowish-orange in color and had a fruity smell. For example, your body is covered in bacteria that eat the dead skin and there are also bacteria living. MALDI-TOF methods are now routinely used for diagnostic procedures in clinical microbiology laboratories. The road to discovering both unknown bacteria given by the instructor was a rocky one; seemingly countless trials and errors later, a conclusion was reached for both. The purpose of this laboratory exercise is to evaluate the student's knowledge and capabilities in identifying micro organism. , lactic acid). UNKNOWN LAB REPORT. Essay on Micro Unknown Lab Report 2051 Words Nov 16, 2011 9 Pages | Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. This is due to the fact that there are only a few basic shapes and physical features commonly seen in the prokaryotic world. Our online microbiology lab trivia quizzes can be adapted to suit your requirements for taking some of the top microbiology lab quizzes. Introduction Bacteria in nature do not exist as pure cultures like the ones that we have used in lab. mirabilis colonies appeared transparent indicating that lactose had not been fermented. Take the test tube labeled indole. When the "Identify now" button is clicked, result would appear in the space below in percentage probabilities. Identification of Unknown Solutions INTRODUCTION In this experiment, the student will determine if a chemical reaction has taken place when two solutions are combined, describe the chemical reaction, and use this information and logic to determine the identity of ten unknown solutions. The General Microbiology I course taught students about many kinds of bacteria. Report on Identification of Mixed Culture Unknown Lab Exercise 33. UNKNOWN LAB REPORT. STARCH AGAR SKIM MILK AGAR SPIRIT BLUE AGAR Unknown A Unknown A Unknown A B. Failure to perform or complete the unknown lab experiments or determine the unknown IDs or turn in a report based on the work done = up to - 75 points off!. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. You need to understand that some tests will give you a result for multiple criteria. Joan McKearnan. Wang said a powerful aspect of the new technique is that it will work with the many other microscopy tools that scientists already have at their disposal. MICROBIOLOGY UNKNOWN LAB REPORT. The purpose of this laboratory exercise is to evaluate the student’s knowledge and capabilities in identifying micro organism. A mark appears next to the selection. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. The unknown specimen for this study was number 107. Print and fill out a copy of the Team Member Evaluation Form from the link above. MATERIALS AND METHODS Sampling Samples for LAB isolation were collected from five. Choose from 500 different sets of microbiology lab identification unknowns flashcards on Quizlet. Procedures 1. Microbiology Lab, flow chart for gram-staining and cocci species. Williamson called to report that a child of a Clark College student enrolled in a microbiology class, BIOL&260, taught by Travis Kibota was ill with Salmonella. Bacteria do not come in pure cultures (except in classroom exercises). For the next several weeks, your sole responsibility will be to identify the two microbes in the test tubes you have been handed. The term bacteria is the plural form of the word bacterium, which represents a single organism. are classified by gram staining. Extract DNA, amplify and sequence one or several phylogenetic marker genes (e. So we just started identification of our unknown bacteria today in lab. This project was created with Explain Everything ™ Interactive Whiteboard for iPad. This study is the determination of the UNKNOWN bacterium that was given by Microbiology LAB Professor. Many pathogenic bacteria exhibit a relatively narrow range of pH over which they will grow. PCR and Sequencing. Some of the characteristics include being able to produce their own. The first procedure that needed to be done was to treak the unknown out on a Trypticase Soy Agar plate, using the T streak method described in the lab manual. An unknown test tube labeled 120 was given out by the lab instructor. The most common bacterial cause of a sore throat is an infection with group A streptococcus. Most identification keys are dichotomous keys which means that each time it branches there will be two equal choices available. This post was most recently updated on August 24th, 2019. Microbiology Lab Cyanobacteria, Protozoans, and Algae Prokaryotic and Eukaryotic Cells As you know, the building blocks of life are cells. Therefore, when working with bacteria you must wear a lab coat, safety glasses and gloves. bacteria identification flow chart somewhat helpful if you were the ambitious one who used an environmental source for your unknown. Bacteria Sampling (formerly Disposable Lab Equipment) should be followed by Gram Staining, then Using a Microscope This work was supported by USDA CSREES and USDA National Institute of Food and Agriculture under two Higher Education Challenge Grant projects: 2008-38411-19055 and 2011-38411-30625. My instructor started this lab by handing me a tube of two unknown bacteria's labeled 116. Newer techniques like real-time PCR, matrix-assisted laser desorption ionization-time of flight mass spectrometry and multiplex-tandem PCR are being increasing employed for the identification of yeast species. Bacterial Shapes Study a “bacteria type” slide illustrating the three shapes of bacteria. The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture. Help! I need to know what this organism is. 5) What is a meniscus? 6) Can density be used as identification for substances in lab? Why or why not? 7) Which was more accurate, your own lab group data or the class data?. What others are saying Unknown Bacteria Identification See more. STARCH AGAR SKIM MILK AGAR SPIRIT BLUE AGAR Unknown A Unknown A Unknown A B. April 29, 2014. Take note: There are no side questions in this test unlike the actual practical exam. Traditionally this is achieved by carrying out labour-intensive and time-consuming biochemical assays. UNKNOWN LAB REPORT. Search by structure, identify chemical synonyms, and find commercial sources. Walt Ream - Thursday lab Note: All MB 303 labs start Week 1, with a no-show drop policy for the class. The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture. [Keep in mind that organisms other than the Enterobacteriaceae and Pseudomonas can cause these infections, so in a real clinical situation other lab tests and cultures for bacteria other than those upon which this lab is based would also be done. A 57 year old female who is diabetic, a smoker, and who 30 days ago had hip replacement surgery presents with pain and signs of inflammation at the surgical site. You will receive an unknown bacterial culture (a gram positive, and a gram negative species, mixed) in March that you must identify, for 60 points. Also all the rules mentioned in the "Identification of Unknown Bacterial Species: General Aspects" chapter apply here. Methods and Materials. Indigenous Unknowns Lab Report Introduction : Normal flora, which make up about 90% of the cells of a human body, are microbes that live and grow on the body without causing disease under normal conditions. The I dentification M atrix shows your assigned unknown with the results you have recorded for it in the. shape, Gram-positive or negative etc. This study is the determination of the UNKNOWN bacterium that was given by Microbiology LAB Professor. This stain is used in the identification of bacillus-shaped. 30 August 2019. Ace your school projects with these 12 featured Prezi presentations and templates. MALDI-TOF methods are now routinely used for diagnostic procedures in clinical microbiology laboratories. finicky bacteria that may not be able to digest the whole red blood cells in blood agar. This approach lacks molecular techniques such as sequencing of genes. Welcome to the Virtual Bacterial Identification Lab. UNKNOWN LAB REPORT. Identification of bacteria 3. An unknown labeled 123 was given out by the lab instructor. from soil, especially beneficial bacteria. Besides utilizing methyl red medium to determine the presence of lactose fermenting bacteria, one may also utilize MacConkey (MAC), EMB (eosin-. The study of microbiology requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. The conclusion drawn from these various tests was that unknown is Enterococcus faecalis. Gram stain For completing the Gram stain we needed Gram stain reagents (crystal violet, Gram's iodine, 95% ethyl alcohol, and safranin), microscope slides and bacterial cultures. pneumoniae is a fastidious bacterium, growing best at 35-37°C with ~5% CO 2 (or in a candle-jar). What is it used for? Bacteria culture tests are used to help diagnose certain types of infections. This is due to the fact that there are only a few basic shapes and physical features commonly seen in the prokaryotic world. This study is the determination of the UNKNOWN bacterium that was given by Microbiology LAB Professor. The purpose of this experiment is to apply the knowledge gained from the entire semester in the Microbiology Lab and apply it to be able to identify bacteria. Iwen, PhD, Associate Director, NPHL For more than 100 years, Robert Koch’s postulate that required in part the cultivation of a pathogen to show a disease/pathogen relationship, was seldom questioned and was considered the basic standard used in clinical diagnostics. Quadrant streak with unknown bacteria Inoculate: TSB broth (for determination of growth),. DISCUSSION. In lab, we have to identify two unknown organisms in a sample of nutrient broth. Methods and Materials. aureus Unknown B Unknown B Unknown B UREA TRYPTONE UREA btilis ureus BROTH BROTH BROTH B. In microbiology laboratory class many techniques were studied to learn how a microorganism will react in certain conditions. Microbiology - Unknown Identification Tests. Neisseria gonorrhoeae is an example of finicky bacteria. What is the overall purpose of this virtual lab? 2. Inoculate blood agar plate with suspected alpha-hemolytic isolates. Differential Staining Techniques Viewing Bacterial Cells. of Microbiology Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Penny Pitman. The first three images are of notes taken by students during a demonstration dissection, showing appropriate detail in the information which was being given as the dissection was performed. Each of these powders can be superficially described as a “dry white powder”, but they have different chemical and physical properties. Introduction The process of identifying bacteria is like solving a mystery; all requiring is to identify the clues. Many pathogenic bacteria exhibit a relatively narrow range of pH over which they will grow. Outcomes: After you complete this lab, you will be able to: Isolate and identify bacteria in an unknown sample. INTRODUCTION. Our teacher didn t really explain much and I m kind of lostI was hoping someone could help I found it is Gram Negative and Rod-Shaped. It produces benefits for many aspects of the research of microorganisms and helps physicians correctly treat patients. View Lab Report - Morphological Unknown Lab Report from BIOL 402 at University of Kansas. Other than the tests previously discussed for the identification of Bacillus cereus, there are several tests that can accompany them to definitively identify the bacterium as Bacillus cereus. Inoculate blood agar plate with suspected alpha-hemolytic isolates. 1: DEMO FLOW CHARTS RECOMMENDED FOR PRE-LAB PREPARATION TO BE PLACED IN YOUR LAB NOTEBOOK. Transcript of IDENTIFICATION of UNKNOWN BACTERIA. Make sure you review the instructions before you do the Gram stain. Joan McKearnan. Gene sequencing can also be used to differentiate between two. Figure 4: Dichotomous key for the identification of lactose non-fermenting enteric bacteria. I had a friend that took microbio in a virtual lab with no instructor, and they seemed really lost in the sauce. Methods and Materials. The purpose of this experiment is for students to understand microbe identification in the context of a clinical situation. docx), PDF File (. In lab, we have to identify two unknown organisms in a sample of nutrient broth. Identification is based upon the labeling of bacteria, parasites, and fungi with appropriate binomial names of Latin or Greek origin. Spring 2013. The purpose of this study was to closely identify unknown bacteria. Studies have shown that of the bacteria found in indoor air, the most common four are: Micrococcus, Staphylococcus, Bacillus, and Pseudomonas. The micro lab website, your textbook, the web and assorted books available in lab will be the reference materials necessary for you to successfully complete the next several weeks of lab work. It is in the lab that most of the testing, culturing, and research that they do occurs. The biochemical tests were: Lactose fermentation, Tryptophan digestion (Indole), Orinithine, methyl red and. Known bacteriophages are used to identify unknown bacteria by observing whether or not the bacteria are lysed. Starting in the early 1970s, Dr. UNKNOWN LAB REPORT. Gram stain For completing the Gram stain we needed Gram stain reagents (crystal violet, Gram's iodine, 95% ethyl alcohol, and safranin), microscope slides and bacterial cultures. ) of an unknown bacterial isolate, be able to use the dichotomous key to determine the possible genus of the unknown. Spring 2013 INTRODUCTION. shape, Gram-positive or negative etc. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. Identifying the Unknown Bacteria. When you’re studying microbiology, you need to know the key differences between the three domains of life, how scientists name and classify organisms, and how scientists identify microorganisms. The test tube used in this study was tube 109. Researcher and biochemist Torkild Visnes in his lab at SINTEF. Correctly handling the specimen Quickly transporting the specimen to the lab. The MicroSEQ ID microbial identification system, based on comparative rDNA sequencing of the 16S region (for bacteria) or the LSU D2 region (for fungi), is a proven method for rapid and accurate microbial identification. Instructions: Identify the organism based on its colonial morphology on its culture medium of choice and its different reactions to the biochemical tests. UNKNOWN LAB REPORT. meningitidis is a fastidious organism, which grows best at 35-37°C with ~5% CO 2 (or in a candle-jar). For example, your body is covered in bacteria that eat the dead skin and there are also bacteria living. With readily available commercial equipment and kits, it is now routine. April 29, 2014. Identifying Unknown Bacteria Lab 1. In addition, an identification of Salmonella or Shigella would usually be confirmed by direct serologic testing as will be described in Lab 17. Example of Bacteria Sample Testing Lab Report - Free download as Word Doc (. coli strains Characteristics of E. The accurate and definitive identification of microorganisms, including bacteria, is one of the cornerstones forming the joint foundation of the fields of microbiology and infectious diseases. Unknown Bacteria Lab Report Introduction The purpose to this lab was to identify an unknown bacteria from a mixed culture provided to us by our instructor. A 57 year old female who is diabetic, a smoker, and who 30 days ago had hip replacement surgery presents with pain and signs of inflammation at the surgical site. What is the best method for isolation and identification of bacteria from soil? I am trying to isolate and identifying the bacterial spp. Microbiology Unknown Lab Report Essay 2396 Words Apr 16, 2012 10 Pages Lab12-Medical Microbiology- Part1– Differential Media/Biochemical Tests, Sp2012 (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab. The compound was identified to be N-Phenysuccinimide, because it had a melting range of 154. Microorganisms sense and adapt to changes in their environment. Introduction Bacteria in nature do not exist as pure cultures like the ones that we have used in lab. The purpose of this laboratory exercise is to evaluate the student’s knowledge and capabilities in identifying micro organism. Study Flashcards On Microbiology Lab Test 1 at Cram. If you have already published your "findings," you will have to retract the article. In his textbook, Campbell Biology, Reece et. Wang said a powerful aspect of the new technique is that it will work with the many other microscopy tools that scientists already have at their disposal. Identification of unknown bacteria using dichotomous key-Part 1 microbiology lab practical information part 1 - Duration: Phenetic identification of bacteria-Part 2 - Duration:. Not long ago, DNA sequencing was a time-consuming, tedious process. Record the microscopic appearance of cells from each culture. Spring 2014 INTRODUCTION It is essential in medicine to be able to identify different microorganisms for diagnosing the cause of various diseases, and determining the action for treatment. Some of these pathways produce unstable acidic products which quickly convert to neutral compounds. “Today we study a tiny subset of bacteria,” said Bo Wang, assistant professor of bioengineering at Stanford. Spring 2013 INTRODUCTION. Biochemical identification of bacteria 1. Comparison of microbial identification methods- traditional methods-molecular methods Related eBooks:. By using X-ray crystallography, we have solved the structure of Escherichia coli SdhE in complex with SdhA to 2. You will find Bergey's Manual of Determinative Microbiology to be a very helpful resource. The following tests were performed on the gram-­‐positive bacteria: 1. However, once they multiply into millions of colonies in a Petri dish they become more of a hazard. INTRODUCTION The identification of an unknown is beneficial in many ways, including the identification of bacteria in diseases, and in research. cerebrospinal fluid (used for the culture of mycobacteria, fungi, bacteria, or bacterial antigen testing) Submit a separate sterile screw-capped tube containing at least 0. Our teacher didn t really explain much and I m kind of lostI was hoping someone could help I found it is Gram Negative and Rod-Shaped. Cinnaminson, NJ, October 24th , 2019 A recent study determines what items in your home have the most germs. Traditionally this is achieved by carrying out labour-intensive and time-consuming biochemical assays. Biology Online - Information in the life sciences in the form of tutorials, references, an online dictionary of biology terms and a biology forum. To accurately examine the characteristics of an individual species, the species must be isolated from the. The compound was identified to be N-Phenysuccinimide, because it had a melting range of 154. Unknown Number 115. Bacteria _ Gram Reaction. Introduction The last of the various diagnostic tests one may perform to identify bacteria is the determination of bacterial nitrate (NO3-) reduction on nitrate medium. Overview: In this experiment, you will combine both spectroscopy and qualitative tests to identify an unknown organic compound. Bacterial Identification Laboratory Exercise Materials from the Virtual Microbiology Classroom. Microbiology. Extract DNA, amplify and sequence one or several phylogenetic marker genes (e. In my case I had a gram negative bacteria. Report for "Unknown" Identification Project Jessica Olivares Francisco Alarcon-Chaidez 12/02/2015 Negative and Positive Identification Of Unknown Bacterial Strains Report Introduction/Purpose The objective of this lab was to classify the unknown bacterial strains provided utilizing a number of biochemical procedures. USING BIOCHEMICAL TESTING TO IDENTIFY. Start studying Identification of Unknown Bacteria Lab (1). In this lab we will focus on using Solubility Tests, Chemical Tests and Spectra Analysis to identify two unknown compounds. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. A culture is a growth of bacterium on artificial nutrient medium or culture medium prepared in the laboratory. A 57 year old female who is diabetic, a smoker, and who 30 days ago had hip replacement surgery presents with pain and signs of inflammation at the surgical site. The growth requirement and the appearance of colonies on media to the naked eye are further criteria to assist the identification of bacteria. Inoculate the broth with the inoculation loop containing the inoculum from the nutrient agar slant. It is a common medium used to culture bacteria because: It is a great enrichment medium for fastidious bacteria. Prokaryotic cells are those cells that do NOT have a nucleus. If you don't attend lab Week 1 you will be dropped from MB 303. Make sure you include the unknown identification on your report along with the compound name for your unknown (i. INTRODUCTION. Introduction Bacteria in nature do not exist as pure cultures like the ones that we have used in lab. All organisms require the major elements. Take the test tube labeled indole. Ace your school projects with these 12 featured Prezi presentations and templates. STARCH AGAR SKIM MILK AGAR SPIRIT BLUE AGAR Unknown A Unknown A Unknown A B. Identifying bacteria can lead to many new discoveries such as new species or the evolution of a known species. By observing the gram-staining of bacteria culture under microscope, if the bacteria cultured stained purple, we observed its morphologwhether it shape cocci or bacilli, if cocci we can further down the hierarchy whether its Staphylococcus or Streptrococcus. Introduction Identification of unknown bacteria will allow the student to review. This is due to the fact that there are only a few basic shapes and physical features commonly seen in the prokaryotic world. What is the overall purpose of this virtual lab? 2. identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. In this paper I will discuss the processes of how I came to find my two unknown bacteria. Example of a Microbiology Unknown Lab Report by Taylor Autry Introduction. Prokaryotic cells are those cells that do NOT have a nucleus. The results of the biochemical tests along with deductive reasoning and elimination led to the identification the unknown bacteria. SCENERIO FOR TODAY'S LAB. This lab was designed for 500 students split into lab sections of 20. Gram positive bacteria: Stain dark purple due to retaining the primary dye called Crystal Violet in the cell wall. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Score the plates according with the scale shown in Figure 1. Start studying Identification of Unknown Bacteria Lab (1). cerebrospinal fluid (used for the culture of mycobacteria, fungi, bacteria, or bacterial antigen testing) Submit a separate sterile screw-capped tube containing at least 0. The road to discovering both unknown bacteria given by the instructor was a rocky one; seemingly countless trials and errors later, a conclusion was reached for both. Each of these powders can be superficially described as a “dry white powder”, but they have different chemical and physical properties. Title This report will cover the identification process of a bacterial sample, Staphylococcus epidermidis,. It is important to always keep in mind that your unknown species is a potential pathogen. Differential Stains: Gram Stain and Acid-fast Stain II. Microbiology courses often include a laboratory activity on the identification of unknown microbes. Identification of Streptococcus pneumoniae by biochemical reactions. BIO 203-604 Introduction. Try to answer these questions without help from notes or books. Label the tube. Due to rapid mutations, bacteria can be found virtually anywhere. A 57 year old female who is diabetic, a smoker, and who 30 days ago had hip replacement surgery presents with pain and signs of inflammation at the surgical site. The instructor asked that each student isolate the two unknown bacteria and then identify each. Excise correct bands from gel and. The conclusion drawn from these various tests was that unknown is Enterococcus faecalis. Our teacher didn t really explain much and I m kind of lostI was hoping someone could help I found it is Gram Negative and Rod-Shaped. Does anyone have any hints? I did find a website that you can put info into it and it will narrow it down, but if anyone has a site that could help?. Only a small percentage, less than 10%, of all known bacteria are pathogenic, or able to cause disease in a susceptible host. This does NOT mean that they are strict aerobes. Melanie Intemann. Quickly memorize the terms, phrases and much more. By observing the gram-staining of bacteria culture under microscope, if the bacteria cultured stained purple, we observed its morphologwhether it shape cocci or bacilli, if cocci we can further down the hierarchy whether its Staphylococcus or Streptrococcus. A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Answer Wiki. I Gram stained it and found Gram+ cocci, and Gram- rods. Identification and Characterization of Streptococcus pneumoniae. Microbiology Bacteria Flow Chart Lab Unknown Identification Effendi #157971024215 – Microbiology Unknown Flow Chart, with 35 More files. LABORATORY RESULTS WORKSHEETS (2 pages) Name Lab Coordinator. Some of these pathways produce unstable acidic products which quickly convert to neutral compounds. You will be given three bacterial cultures and asked to identify each to the level of genus. Observations: The unknown was yellowish-orange in color and had a fruity smell. The results of the biochemical tests along with deductive reasoning and elimination led to the identification the unknown bacteria. Starting in the early 1970s, Dr. docx), PDF File (. apiweb™ Loading. Inoculate blood agar plate with suspected alpha-hemolytic isolates. We can collect and grow them in specially prepared petri dishes. Gram Stain B. The microscope is a very important tool in microbiology, but there are limitations when it comes to using one to observe cells in general and bacterial cells in particular. DISCUSSION.